Correlation of Microplastic Levels with Erythrocyte Profiles in Wistar Rats Orally Exposed to Polyethylene Microplastics
DOI:
https://doi.org/10.58954/epj.v6i1.435Keywords:
Microplastics, Erythrocytes, Oxidative stress, Polyethylene, Rattus norvegicusAbstract
Polyethylene microplastics are widely used in single-use products and can enter the body through contaminated food or beverages. After ingestion, these particles may cross the intestinal barrier and reach systemic circulation. Their presence in blood is suspected to induce oxidative stress through increased reactive oxygen species, potentially affecting erythrocyte membrane integrity. Erythrocytes are essential for oxygen transport, and structural damage may reduce their flexibility and lead to morphological abnormalities. This study aimed to evaluate the correlation between microplastic counts in blood and erythrocyte profiles in Wistar rats exposed orally to polyethylene microplastics. A quantitative experimental study with a post-test only control group design was conducted. Male Wistar rats were assigned to one control group and three exposure groups receiving different microplastic doses for four weeks. Microplastics were identified using light microscopy and Fourier Transform Infrared spectroscopy, while erythrocyte profiles were assessed through peripheral blood smears and hematological examination. Microplastics were detected in exposed groups, accompanied by several abnormal erythrocyte forms. However, no significant differences or correlations were observed between microplastic counts and erythrocyte parameters. Oral exposure to polyethylene microplastics did not significantly alter erythrocyte profiles, although potential morphological effects cannot be excluded.
References
Abdel-Zaher, S., Mohamed, M. S., & Sayed, A. E. D. H. (2023). Hemotoxic effects of polyethylene microplastics on mice. Frontiers in Physiology, 14. https://doi.org/10.3389/fphys.2023.1072797
Aridya, N. D., Yuniarti, E., Atifah, Y., & Farma, S. A. (2023). The Differences Erythrocyte and Hemoglobin Levels of Biology Students and Sports Students Universitas Negeri Padang. Jurnal Serambi Biologi, 8(1), 38–43.
Badan Pusat Statistik. (2021). BPS: 270,20 Juta Penduduk Indonesia Hasil SP2020. In Badan Pusat Statistik.
Budiarti, E. C. (2021). Identifikasi Mikroplastik pada Feses Manusia. Environmental Pollution Journal, 1(2). https://journalecoton.id/index.php/epj
Candra, K. A. (2023). Identifikasi Mikroplastik pada Air Sungai Akibat Limbah Pabrik Daur Ulang Plastik di Gresik dan Sidoarjo. Environmental Pollution Journal, 3(3), 833–840. https://doi.org/10.58954/epj.v3i3.141
Deng, Y., Zhang, Y., Lemos, B., & Ren, H. (2017). Tissue accumulation of microplastics in mice and biomarker responses suggest widespread health risks of exposure. Scientific Reports, 7(1), 46687. https://doi.org/10.1038/srep46687
Farag, A. A., Youssef, H. S., Sliem, R. E., Gazzar, W. B. El, Nabil, N., Mokhtar, M. M., Marei, Y. M., Ismail, N. S., Radwaan, S. E., Badr, A. M., & Sayed, A. E.-D. H. (2023). Hematological consequences of polyethylene microplastics toxicity in male rats: Oxidative stress, genetic, and epigenetic links. Toxicology, 492, 153545. https://doi.org/10.1016/j.tox.2023.153545
Hamed, M., Osman, A. G. M., Badrey, A. E. A., Soliman, H. A. M., & Sayed, A. E.-D. H. (2021). Microplastics-Induced Eryptosis and Poikilocytosis in Early-Juvenile Nile Tilapia (Oreochromis niloticus). Frontiers in Physiology, 12. https://doi.org/10.3389/fphys.2021.742922
Hartini, A. S. A., & Dewi, R. S. (2021). Identifikasi Kandungan Mikroplastik pada Ikan dan Air Hilir Sungai Brantas. Environmental Pollution Journal, 1(2). https://journalecoton.id/index.php/epj
Higgins, G. A., & Sellers, E. M. (1994). Antagonist-precipitated opioid withdrawal in rats: Evidence for dissociations between physical and motivational signs. Pharmacology Biochemistry and Behavior, 48(1), 1–8. https://www.sciencedirect.com/science/article/abs/pii/0091305794904898
Husnalia, N., Nugroho, S., & Adnan, F. (2023). Analisis Keterkaitan Kelimpahan Mikroplastik dengan Sampah Plastik pada Sungai Mahakam di Desa Sebulu Modern Kecamatan Sebulu. Jurnal Teknologi Lingkungan Universitas Mulawarman, 7(2). https://doi.org/10.30872/jtlunmul.v7i2.10827
Kadac-Czapska, K., Ośko, J., Knez, E., & Grembecka, M. (2024). Microplastics and Oxidative Stress—Current Problems and Prospects. Antioxidants, 13(5), 579. https://doi.org/10.3390/antiox13050579
Khoironi, A., Huda, K., Hambyah, I., & Dianratri, I. (2021). Pengaruh Mikroplastik Polietilen dan Oxo-degradable (Oxium) Pada Pertumbuhan Mikroalga Tetraselmis chuii. CALIENTE PRESS.
Mardalisa, Fatwa, E. B., Yoswaty, D., Feliatra, Effendi, I., & Amin, B. (2021). Isolasi dan Identifikasi Bakteri Indigenous Pendegradasi Plastik dari Perairan Laut Dumai Provinsi Riau Isolation and Identification of Indigenous Plastic-Degrading Bacteria from Dumai ’ s Ocean Water of Riau Province. Jurnal Ilmu Perairan (Aquatic Science), 9(1), 77–85.
Melindo, H. L., Riani, E., Wiradika, M., & Sinambela, C. (2022). Mikroplastik dalam Budidaya Teripang Pasir (Holothuria scabra): Studi Kasus Pulau Pasaran, Bandar Lampung. Coastal and Ocean Journal, 6, 73–80. https://journal.ipb.ac.id/index.php/coj;
Pan, C., Wu, Y., Hu, S., Li, K., Liu, X., Shi, Y., Lin, W., Wang, X., Shi, Y., Xu, Z., Wang, H., & Chen, H. (2023). Polystyrene microplastics arrest skeletal growth in puberty through accelerating osteoblast senescence. Environmental Pollution, 322. https://doi.org/10.1016/j.envpol.2023.121217
Preda, O. T., Vlasceanu, A. M., Andreescu, C. V., Tsatsakis, A., Mezhuev, Y., Negrei, C., & Baconi, D. L. (2024). Health Implications of Widespread Micro- and Nanoplastic Exposure: Environmental Prevalence, Mechanisms, and Biological Impact on Humans. In Toxics (Vol. 12, Issue 10). Multidisciplinary Digital Publishing Institute (MDPI). https://doi.org/10.3390/toxics12100730
Rajendran, D., & Chandrasekaran, N. (2023). Journey of micronanoplastics with blood components. In RSC Advances (Vol. 13, Issue 45, pp. 31435–31459). Royal Society of Chemistry. https://doi.org/10.1039/d3ra05620a
Thiagarajan, P., Parker, C. J., & Prchal, J. T. (2021). How Do Red Blood Cells Die? In Frontiers in Physiology (Vol. 12). Frontiers Media S.A. https://doi.org/10.3389/fphys.2021.655393
Ummah, N., Humaidah, N., & Suryanto, D. (2023). Analisis dan Prediksi Dampak Mikroplastik pada Unggas Air (Article Review). Dinamika Rekasatwa: Jurnal Ilmiah (e-Journal), 6(2). https://jim.unisma.ac.id/index.php/fapet/article/view/20109
Yong, C., Valiyaveettil, S., & Tang, B. (2020). Toxicity of Microplastics and Nanoplastics in Mammalian Systems. International Journal of Environmental Research and Public Health, 17(5), 1509. https://doi.org/10.3390/ijerph17051509
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